منابع مشابه
Studies on the secondary structure of nuclear ribonucleic acids.
Rat liver nuclei were separated into two fractions, the nucleolus and the nucleoplasm, by a combined salt-enzymic extraction. RNA was purified from both sources and analysed on sucrose density gradients. In both fractions a prominent heterogeneous RNA class with mean sedimentation coefficient of 18S was found. This material was analysed by measuring the rate of reaction with formaldehyde, the u...
متن کاملOn the Structure of Ribonucleic Acids
Early concepts of the structure of ribonucleic acid (RNA) were based largely upon the isolation of the nucleotides of adenine, guanine, uracil, and cytosine from alkali-hydrolyzed RNA (1). Since these were regarded as being single compounds phosphorylated in the 3’ position, and since the acid-stable ribose-5-phosphate (and its pyrimidine derivatives) could not be found in acid-hydrolyzed RNA, ...
متن کاملOn the Structure of Ribonucleic Acids
The complete hydrolysis of ribonucleic acids (RNA) to their constituent mononucleotides, while necessary for determining the composition and also indicative of the nature of the phosphoryl attachments, fails to give any information as to the sequences or subgroup structures existing in the parent molecule. The need and qualifications for a method which will bring about a reproducible degree of ...
متن کاملThe secondary structure of ribosomal ribonucleic acid in solution.
1. The u.v.-absorption spectrum of ribosomal RNA from rabbit reticulocytes was studied as a function of temperature at different pH values. The changes in the spectrum over the range 220-320mmu were interpreted on the basis of the assumption that the effect of denaturation and ionization are additive. The results suggest that in neutral salt solutions the secondary structure of the ribosomal RN...
متن کاملRibonucleic Acids: Capillary Electrophoresis
With the introduction of capillary electrophoresis (CE), a new generation of electrophoretic techniques has seen the light of day. The scientiRc literature today describes a large number of applications of this powerful analytical technique in the analysis of nucleic acids. For nucleic acids, as for most other analytes, CE offers signiRcant advantages over many of the conventional electrophoret...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1959
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.45.4.482